Tag Archives: Cyp450

Neonate Maturation – Cytochrome P450

VLA Comment:  Clearly cyp 450 family of enzymes are “maturing”.  Many vaccine excipients given to neonates and children (such as formaldahyde (cyp4501A2), polysorbate 80 (cyp4503A4) cannot be metabolized, therefore poisoning the neonate, infant, child potentially at every 2 month well baby visit.

Some CYP450 enzymes are active in utero while others do not demonstrate activity until some time after birth. When corrected for weight the content of CYP enzymes in fetal livers is 30–60% of adult values. The link below has a table that indicates maturation times in some cyp 450 enzymes.

Neonatal maturation of Cyp 450 enzymes study*

Excerpt: Maturation rates are difficult to generalize and enzyme‐specific information needs to be determined for an accurate estimate of drug metabolism 7. Polymorphisms, diseases such as sepsis and complex surgery can all increase the variability of drug metabolism 32. A diet based on infant formula rather than breast milk 14 and antenatal exposure to cigarette smoke 33 can increase the rate of CYP enzyme development. Further, the routes of metabolism seen in adults may not be mirrored in neonates due to the activity or inactivity of particular CYP enzymes 3, summarized in Table 1. (click link above for table)

The Ontogeny of Cytochrome P450 Enzyme Activity and Protein Abundance in Conventional Pigs in Support of Preclinical Pediatric Drug Research

CYP450 enzymes are the most important phase I drug metabolizing enzymes, located on the smooth endoplasmic reticulum and highly expressed in liver compared to other tissues in pigs (Antonovic and Martinez, 2011; Nielsen et al., 2017). They metabolize between 70 and 80% of the human drugs used, most frequently by hydroxylation (Matalová et al., 2016). The ontogeny of the human CYP450 enzymes is rather well known. For example CYP2C9 enzyme activity increases rapidly after birth reaching adult values around 3 months of age, whereas CYP1A2 reaches adult values around 10 years of age (Alcorn and McNamara, 2002; Johnson et al., 2006; Matalová et al., 2016).

Click here for study

Drugs metabolized by 1A2  (as an example), such as formaldahyde in vaccines

Effects of Heavy Metals in Vaccines (pharmacogenetics)

Aside from the known DEPOPULATION trends such social programs to reduce births, abortion legislation, reduced male sperm count via toxic agriculture (50% reduction as early as 1945), the increasing need for in vitro derived pregnancies, iatrogenic deaths, etc, the worldwide establishment is actually “poisoning” all neonates and infants under 3 years old.

The Journal of Pediatric Pharmacology and Therapeutics

“Developmental Pharmacokinetics in Pediatric Populations”

Hong Lu, PhD and Sara Rosenbaum, PhD

*Ped cyp enzymes  (see graph)  (very important study of the immaturity of cyp 450 superfamilies in infants and children. Synopsis:  Infants do not have a mature liver or liver enzyme function such as Cytochrome P450 and its various metabolites until the age of three years old. Hence upwards of 36 vaccine doses by 18 months old containing the above excipients are poisoning the world’s emerging humanity. DOWNLOAD STUDY

Detoxification mechanism (Fish Study/Liver)Effect Metals on Cyp 450PHD_Thesis_Eng(1)

CDC vaccine excipients

Failure of Infants to metabolize due to immature Liver Cyp450 system of enzymes until 3 years old.

The Effect Of Heavy Metal on Hepatic Chromosome P450

The results of our experiments have demonstrated that heavy metal ions react with cytochrome P450 dependent enzyme system of fish species leading to changes in confirmation of protein structure they resolve in inhibition of activity of enzymes involved in biotransformation, decrease in detoxifying enzymes. In conclusion the difference changes in enzyme activity and cytochrome P450 content induce by the various heavy metal treatments may also be explained by the living and feeding habits of individual fish species.

Like with all living organism, the body of the fish has a quite conserve protein systems providing molecular defense to insure metabolism and elimination of the foreign material. The main region of metabolism of pollutants entering into the fish body is the liver. Due to biotransformation processes these substances are converted to other compounds that can be eliminated from the body thereby becoming less toxic. The hepatic detoxifying enzymes, the cytochrome P450 dependent mixed function monooxygenases, have an important role in this process.  P450 enzyme activity in the body is not constant.  Foreign substances, Xenobiotic or some endogenous regulating molecules are able to increase (inducers) or decrease (inhibitors) the activity of cytochrome P450 enzymes.

Aluminum + Cytochrome P450 -Study: Suppressive effect of accumulated aluminum trichloride on the hepatic microsomal cytochrome P450 enzyme system in rats.

Vaccine Excipientsf/CDC Vaccine Excipient Chart

  • Which ones need a mature Cyp 450 to metabolize out of the body?

 

  • aluminum hydroxide
  • aluminum phosphate
  • ammonium sulfate
  • amphotericin B
  • animal tissues: pig blood, horse blood, rabbit brain,
  • dog kidney, monkey kidney,
  • chick embryo, chicken egg, duck egg
  • calf (bovine) serum
  • beta propiolactone
  • fetal bovine serum
  • formaldehyde
  • formalin
  • gelatin
  • glycerol
  • human diploid cells (originating from aborted human fetal tissue)
  • hydrolized gelatin
  • mercury thimerosal (thiomersal, Merthiolate®)
  • monosodium glutamate (MSG)
  • neomycin
  • neomycin sulfate
  • phenol red indicator
  • phenoxyethanol
  • potassium diphosphate
  • potassium monophosphate
  • polymyxin B
  • polysorbate 20
  • polysorbate 80
  • porcine (pig) pancreatic hydrolysate of casein
  • residual MRC5 proteins
  • sorbitol
  • squalene
  • sucrose
  • tri(n)butylphosphate,
  • VERO cells, a continuous line of monkey kidney cells, and
  • washed sheep red blood

 

VLA Conclusion:  Aside from the known DEPOPULATION trends such social programs to reduce births, abortion legislation, reduced male sperm count via toxic agriculture (50% reduction as early as 1945), the increasing need for in vitro derived pregnancies, iatrogenic deaths, etc, the worldwide establishment is actually “poisoning” all neonates and infants under 3 years old.

The Journal of Pediatric Pharmacology and Therapeutics“Developmental Pharmacokinetics in Pediatric Populations” Hong Lu, PhD and Sara Rosenbaum, PhD

*Ped cyp enzymes  (see graph)  (very important study of the immaturity of cyp 450 superfamilies in infants and children. Synopsis:  Infants do not have a mature liver or liver enzyme function such as Cytochrome P450 and its various metabolites until the age of three years old. Hence upwards of 36 vaccine doses by 18 months old containing the above excipients are poisoning the world’s emerging humanity. DOWNLOAD STUDY

 

 

 

 

 

Pharmacokinetics…aluminum adjuvant Pertussis Vaccine and Allergies

Pharmacokinetics and Differential Regulation of Cytochrome P450 Enzymes in Type 1 Allergic Mice

“We investigated hepatic P450 metabolism and drug pharmacokinetics in type 1 allergic mice induced by ovalbumin (OVA) emulsified with aluminum and inactive Bordetella pertussis. To further propose a possible key factor in drug-allergic disease interactions, we examined the inhibitory effect of NO, a marker of allergic conditions on the activities of major P450 isoforms”.

Read study…

Magic Mushrooms and some individual’s inability to metabolize-causing psychosis

Magic Mushrooms and some individual’s inability to metabolize-causing psychosis, albeit temporary

Magic Mushrooms:  Some individuals are not able to metabolize-causing psychosis, albeit temporary and not to be confused with “mental illness”.

Indolealkylamines: Biotransformations and Potential Drug–Drug Interactions (STUDY)

Increased knowledge in this area may advance our understanding of individual vulnerability to and/or protection from illicit drugs of abuse (). This review, therefore, aims to describe our current understanding of the metabolism  READ STUDY…

 

Pharmacogenetics: Cytochrome P450 in Pediatric Population

The various factors that impact pharmacokinetics
and pharmacodynamics mature towards adult values at different rates, thus requiring continual modification of drug dose regimens in neonates, infants, and children.

Read Study Pediatric Population study Cyp 450.