CRISPR gene editing in human embryos wreaks chromosomal mayhem
Three studies showing large DNA deletions and reshuffling heighten safety concerns about heritable genome editing.
The changes are the result of DNA-repair processes harnessed by genome-editing tools. CRISPR–Cas9 uses a small strand of RNA to direct the Cas9 enzyme to a site in the genome with a similar sequence. The enzyme then cuts both strands of DNA at that site, and the cell’s repair systems heal the gap.
The researchers used CRISPR–Cas9 to create mutations in the POU5F1 gene, which is important for embryonic development. Of 18 genome-edited embryos, about 22% contained unwanted changes affecting large swathes of the DNA surrounding POU5F1. They included DNA rearrangements and large deletions of several thousand DNA letters — much greater than typically intended by researchers using this approach.
EXCERPTS: But the Stanford team that developed PAC-MAN needed an effective way to deliver it to the lung, where COVID-19 often does the most damage. VLA COMMENT: Our research shows that the reason the LUNGS seem to be damaged by Covid-19 is because it is a NANO VIRUS. Studies show that the use of Nanotechnology in humans have a consistent problem…it damages the Lungs.
Science daily paperHealth Risks Of Nanotechnology: How Nanoparticles Can Cause Lung Damage, And How The Damage Can Be Blocked.